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Extraction of volatile oil and its saponins from Bupleurum Bupleuri by supercritical CO_2

author: Popularity:- date:2017/2/13 16:00:32

        The technology of supercritical CO2 extraction of volatile oil and saponins from Bupleurum Bupleurum was studied. Methods: the effects of pressure, temperature, time, flow rate and entrainer on the yield were mainly discussed. The best conditions of supercritical CO2 extraction of volatile oil and saponin from Radix Bupleuri were determined. The supercritical CO2 extraction of volatile oil was compared with the traditional steam distillation method. At the same time, the volatile oil was separated and identified by GC/MS method. Results: the extraction pressure of volatile oil was 2 0MPa, the temperature was 30 degrees, the pressure of I was 12MPa, the temperature was 65 degrees, the pressure of the analyzer was 6MPa, the temperature was 40 degrees, the extraction time was 4h, and the CO2 flux was 10~2 0kg / H-1 per 1kg raw material. The extraction pressure of Bupleurum saponin is 30MPa, the temperature is 65 degrees, the I pressure of the analytical kettle is 12MPa, the temperature is 55 degrees, the pressure of the analyzer is 6MPa, the temperature is 43 degrees, the extraction time is 3h, and the CO2 flux is 20~2 5kg / H-1 per 1kg raw material. Conclusion: the supercritical CO2 extraction of volatile oil is superior to the traditional method, which shows that the yield is greatly improved and the extraction time is short. The volatile oil by 22 chemical composition of hexanal. The saponins of Bupleurum Bupleurum can be put forward by adding the entrainer such as ethanol and increasing the pressure and temperature.


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